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lineweaver−burk plots  (GraphPad Software Inc)
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Lineweaver−Burk Plots, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pro elisa analysis software  (Molecular Devices LLC)
96
Molecular Devices LLC pro elisa analysis software
Pro Elisa Analysis Software, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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switchbuild software  (Dynamic Biosensors)
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Dynamic Biosensors switchbuild software
Switchbuild Software, supplied by Dynamic Biosensors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aid elispot software  (AID Diagnostika)
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prism version 5.0 windows  (GraphPad Software Inc)
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GraphPad Software Inc prism version 5.0 windows
Prism Version 5.0 Windows, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa  (GraphPad Software Inc)
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GraphPad Software Inc elisa
Elisa, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p. multocida romph elisa  (MedCalc Software Ltd)
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MedCalc Software Ltd p. multocida romph elisa
Details of the <t> P. multocida </t> strains used in this study
P. Multocida Romph Elisa, supplied by MedCalc Software Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elispot 2.5 software  (Cell Technology Inc)
90
Cell Technology Inc elispot 2.5 software
Details of the <t> P. multocida </t> strains used in this study
Elispot 2.5 Software, supplied by Cell Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elispot software system  (Carl Zeiss)
90
Carl Zeiss elispot software system
A ) N2a cells and B ) ScN2a cells were inoculated for 1.5 hours with increasing dilutions of PrP 27–30 . The infective media was removed and replaced with regular DMEM for an additional 3 hours before cells were repeatedly washed with PBS and 20,000 cells attached per well of an <t>ELISPOT</t> plate. Cells were examined via the nSCA and mSCA. Positive result is defined as >30 spots/20,000 cells. <t>The</t> <t>software</t> used to count spots (infected cells) cannot count accurately above 1000 spots so a dose response curve is not observed with the mSCA for the ScN2a cells inoculated with PrP 27–30 . Error bars represent SD; n = 2.
Elispot Software System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunospot v 5 1 software  (Cellular Technology Ltd)
97
Cellular Technology Ltd immunospot v 5 1 software
A ) N2a cells and B ) ScN2a cells were inoculated for 1.5 hours with increasing dilutions of PrP 27–30 . The infective media was removed and replaced with regular DMEM for an additional 3 hours before cells were repeatedly washed with PBS and 20,000 cells attached per well of an <t>ELISPOT</t> plate. Cells were examined via the nSCA and mSCA. Positive result is defined as >30 spots/20,000 cells. <t>The</t> <t>software</t> used to count spots (infected cells) cannot count accurately above 1000 spots so a dose response curve is not observed with the mSCA for the ScN2a cells inoculated with PrP 27–30 . Error bars represent SD; n = 2.
Immunospot V 5 1 Software, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p54-elisa (a)  (GraphPad Software Inc)
90
GraphPad Software Inc p54-elisa (a)
A ) N2a cells and B ) ScN2a cells were inoculated for 1.5 hours with increasing dilutions of PrP 27–30 . The infective media was removed and replaced with regular DMEM for an additional 3 hours before cells were repeatedly washed with PBS and 20,000 cells attached per well of an <t>ELISPOT</t> plate. Cells were examined via the nSCA and mSCA. Positive result is defined as >30 spots/20,000 cells. <t>The</t> <t>software</t> used to count spots (infected cells) cannot count accurately above 1000 spots so a dose response curve is not observed with the mSCA for the ScN2a cells inoculated with PrP 27–30 . Error bars represent SD; n = 2.
P54 Elisa (A), supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa assays  (GraphPad Software Inc)
90
GraphPad Software Inc elisa assays
A ) N2a cells and B ) ScN2a cells were inoculated for 1.5 hours with increasing dilutions of PrP 27–30 . The infective media was removed and replaced with regular DMEM for an additional 3 hours before cells were repeatedly washed with PBS and 20,000 cells attached per well of an <t>ELISPOT</t> plate. Cells were examined via the nSCA and mSCA. Positive result is defined as >30 spots/20,000 cells. <t>The</t> <t>software</t> used to count spots (infected cells) cannot count accurately above 1000 spots so a dose response curve is not observed with the mSCA for the ScN2a cells inoculated with PrP 27–30 . Error bars represent SD; n = 2.
Elisa Assays, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa assays/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
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Image Search Results


Details of the P. multocida strains used in this study

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: Details of the P. multocida strains used in this study

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: Isolation

A phylogenetic tree based on the ompH gene sequences of the P. multocida strain F39 and other P. multocida strains of different serotypes or from different hosts.

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: A phylogenetic tree based on the ompH gene sequences of the P. multocida strain F39 and other P. multocida strains of different serotypes or from different hosts.

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques:

SDS-PAGE and western blotting analysis of expressed and purified rOmpH. A) SDS-PAGE analysis of expressed and purified rOmpH. Lane M, protein molecular mass markers; lane 1, a sonicated whole-cell lysate of pET32a-ompH/BL21; lane 2, a sonicated whole-cell lysate of induced pET32a-ompH/BL21; lane 3, purified rOmpH. B) A western blot of purified rOmpH. Lane M, protein molecular mass markers; lane 1, purified rOmpH.

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: SDS-PAGE and western blotting analysis of expressed and purified rOmpH. A) SDS-PAGE analysis of expressed and purified rOmpH. Lane M, protein molecular mass markers; lane 1, a sonicated whole-cell lysate of pET32a-ompH/BL21; lane 2, a sonicated whole-cell lysate of induced pET32a-ompH/BL21; lane 3, purified rOmpH. B) A western blot of purified rOmpH. Lane M, protein molecular mass markers; lane 1, purified rOmpH.

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: SDS Page, Western Blot, Purification, Sonication

ROC analysis of the rOmpH ELISA. A) ROC curves based on results of the rOmpH ELISA ( n =115). B) The relation of ROC-based estimates of test sensitivity and specificity with the rOmpH ELISA cutoffs. C) An interactive dot diagram based on rOmpH ELISA outcomes in relation to the MAT (MAT negative=0 and MAT positive=1).

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: ROC analysis of the rOmpH ELISA. A) ROC curves based on results of the rOmpH ELISA ( n =115). B) The relation of ROC-based estimates of test sensitivity and specificity with the rOmpH ELISA cutoffs. C) An interactive dot diagram based on rOmpH ELISA outcomes in relation to the MAT (MAT negative=0 and MAT positive=1).

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: Enzyme-linked Immunosorbent Assay

Comparison of the indirect ELISA and the MAT

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: Comparison of the indirect ELISA and the MAT

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: Comparison, Indirect ELISA, Enzyme-linked Immunosorbent Assay

The detection rate of anti- P. multocida antibodies in serum samples (collected during 2015) from healthy-looking ducks

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: The detection rate of anti- P. multocida antibodies in serum samples (collected during 2015) from healthy-looking ducks

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques:

A ) N2a cells and B ) ScN2a cells were inoculated for 1.5 hours with increasing dilutions of PrP 27–30 . The infective media was removed and replaced with regular DMEM for an additional 3 hours before cells were repeatedly washed with PBS and 20,000 cells attached per well of an ELISPOT plate. Cells were examined via the nSCA and mSCA. Positive result is defined as >30 spots/20,000 cells. The software used to count spots (infected cells) cannot count accurately above 1000 spots so a dose response curve is not observed with the mSCA for the ScN2a cells inoculated with PrP 27–30 . Error bars represent SD; n = 2.

Journal: PLoS ONE

Article Title: Anti-Prion Drug mPPIg5 Inhibits PrP C Conversion to PrP Sc

doi: 10.1371/journal.pone.0055282

Figure Lengend Snippet: A ) N2a cells and B ) ScN2a cells were inoculated for 1.5 hours with increasing dilutions of PrP 27–30 . The infective media was removed and replaced with regular DMEM for an additional 3 hours before cells were repeatedly washed with PBS and 20,000 cells attached per well of an ELISPOT plate. Cells were examined via the nSCA and mSCA. Positive result is defined as >30 spots/20,000 cells. The software used to count spots (infected cells) cannot count accurately above 1000 spots so a dose response curve is not observed with the mSCA for the ScN2a cells inoculated with PrP 27–30 . Error bars represent SD; n = 2.

Article Snippet: The plastic casing at the back of the elispot plate was then removed and the wells washed a further two times with 160 µl dH 2 O. PrP Sc positive cells were detected and quantified using the Zeiss Elispot software system and Zeiss AX-10 imager (Zeiss).

Techniques: Enzyme-linked Immunospot, Software, Infection