Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: Details of the P. multocida strains used in this study

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: Isolation

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: A phylogenetic tree based on the ompH gene sequences of the P. multocida strain F39 and other P. multocida strains of different serotypes or from different hosts.

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques:

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: SDS-PAGE and western blotting analysis of expressed and purified rOmpH. A) SDS-PAGE analysis of expressed and purified rOmpH. Lane M, protein molecular mass markers; lane 1, a sonicated whole-cell lysate of pET32a-ompH/BL21; lane 2, a sonicated whole-cell lysate of induced pET32a-ompH/BL21; lane 3, purified rOmpH. B) A western blot of purified rOmpH. Lane M, protein molecular mass markers; lane 1, purified rOmpH.

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: SDS Page, Western Blot, Purification, Sonication

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: ROC analysis of the rOmpH ELISA. A) ROC curves based on results of the rOmpH ELISA ( n =115). B) The relation of ROC-based estimates of test sensitivity and specificity with the rOmpH ELISA cutoffs. C) An interactive dot diagram based on rOmpH ELISA outcomes in relation to the MAT (MAT negative=0 and MAT positive=1).

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: Comparison of the indirect ELISA and the MAT

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques: Comparison, Indirect ELISA, Enzyme-linked Immunosorbent Assay

Journal: The Journal of Veterinary Medical Science

Article Title: Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

doi: 10.1292/jvms.17-0124

Figure Lengend Snippet: The detection rate of anti- P. multocida antibodies in serum samples (collected during 2015) from healthy-looking ducks

Article Snippet: The ROC was set up to determine the cutoff value of the P. multocida rOmpH ELISA in MedCalc for Windows, version 9.2.0.1 (MedCalc Software, Ostend, Belgium).

Techniques:

Journal: PLoS ONE

Article Title: Anti-Prion Drug mPPIg5 Inhibits PrP C Conversion to PrP Sc

doi: 10.1371/journal.pone.0055282

Figure Lengend Snippet: A ) N2a cells and B ) ScN2a cells were inoculated for 1.5 hours with increasing dilutions of PrP 27–30 . The infective media was removed and replaced with regular DMEM for an additional 3 hours before cells were repeatedly washed with PBS and 20,000 cells attached per well of an ELISPOT plate. Cells were examined via the nSCA and mSCA. Positive result is defined as >30 spots/20,000 cells. The software used to count spots (infected cells) cannot count accurately above 1000 spots so a dose response curve is not observed with the mSCA for the ScN2a cells inoculated with PrP 27–30 . Error bars represent SD; n = 2.

Article Snippet: The plastic casing at the back of the elispot plate was then removed and the wells washed a further two times with 160 µl dH 2 O. PrP Sc positive cells were detected and quantified using the Zeiss Elispot software system and Zeiss AX-10 imager (Zeiss).

Techniques: Enzyme-linked Immunospot, Software, Infection

Journal: Cellular and Molecular Immunology

Article Title: Naturally occurring spike mutations influence the infectivity and immunogenicity of SARS-CoV-2

doi: 10.1038/s41423-022-00924-8

Figure Lengend Snippet: Substitution of S982A and L18F weakens the generation of binding antibodies. The area under the curve (AUC) of anti-spike ( A ) and anti-RBD ( B ) IgG induced by the 19 designed groups of DNA immunogens ( n = 3 for each group). The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8. Data were analyzed for statistical significance using the unpaired Student’s t test. Data are shown as the mean with SD in each group. Dotted black lines indicate the limit of quantification (LOQ). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay